Custom NGS Select by Seq ID of Additional NGS Representatives by Group - AbXtract

Category Paths

Follow one of these paths in the Orion user interface, to find the floe.

  • Solution-based/Biologics/Antibody Design

  • Role-based/Bioinformatician

  • Role-based/Biologist

  • Product-based/AbXtract


Input AbXtract dataset(s) and also a separate input file (Excel, TSV, CSV), the latter of which is formatted as a sequence ID (seq_id) in the first column and the number of full-length sequences desired by region of interest (cluster or ROI) in the second column. No header should be provided. All additional full-length sequences that map to the same cluster or ROI (user-specified) are written to a separate dataset.

Promoted Parameters

Title in user interface (promoted name)

NGS Key SELECTION Parameters

Metrics for Ranking (picking_choices): Place metrics in order of ranking (if nothing, ranks by full-length count)

  • Required

  • Type: string

  • Default: [‘ROI Percent, Final Round Only’, ‘Full Length (Corrects for Illumina or PacBio), Percent’, ‘Liabilities Both Chains’, ‘Liabilities CDR3_2’]

  • Choices: [‘Full Length (Corrects for Illumina or PacBio), Count’, ‘Full Length (Corrects for Illumina or PacBio), Percent’, ‘ROI Count, Final Round Only’, ‘ROI Percent, Final Round Only’, ‘ROI Fold Enrichment, Final Round Only’, ‘ROI Log2 Enrichment, Final Round Only’, ‘Liabilities Both Chains’, ‘Liabilities Chain_2’, ‘Liabilities Chain_1’, ‘Liabilities CDR1_1’, ‘Liabilities CDR2_1’, ‘Liabilities CDR3_1’, ‘Liabilities CDR1_2’, ‘Liabilities CDR2_2’, ‘Liabilities CDR3_2’, ‘ROI Count, Early Round Only’, ‘ROI Percent, Early Round Only’, ‘Cluster Count (e.g. unique sequences per cluster)’, ‘Cluster Percent (e.g. unique rep per cluster)’]