Tutorials

Tutorials for AbXtract by Specifica

Contents:

• Tutorial 1: NGS Pipeline with Custom Interactive Selection (PacBio), In-Vitro Library
  • Background
  • STEP 1 - Log in to Orion, Set Up Directory, Locate Tutorial Files
  • STEP 2 - Select the ‘NGS Pipeline’ Floe
  • STEP 3 - Prepare PacBio Run and Start Job
  • STEP 4 - Open the Floe Report to Get a General Idea About the Population
  • STEP 5 - Activate the Dataset
  • STEP 6 - Plot Relative Abundance Versus Desired Overlap Population and Select Population with Analyze Tool
  • STEP 7 - Select the Enriched Population Using the Analyze Tool and Send to the Automated Top Lead Selection Floe
  • STEP 8 - Subset the Number of Fields for Output and Download CSV
• Tutorial 2: NGS Pipeline with Automated Top Lead Selection (PacBio), In-Vitro Library
  • Background
  • STEP 1 - Login to Orion, Set-Up Directory, Locate Tutorial Files
  • STEP 2 - Select the ‘NGS Pipeline with Automated Lead Selection’ Floe
  • STEP 3 - Prepare PacBio Run and Start Job
  • STEP 4 - Open the Floe Report to Get a Detailed Understanding of the Selected or ‘Picked’ Population
  • STEP 5 - Select Populations with Net Negative Charge of the CDRs
  • STEP 6 - Subset the Output Fields
  • STEP 7 - Download the CSV
• Tutorial 3: NGS and Sanger Pipeline with Automated Top Lead Selection (PacBio), In-Vitro Library
  • Background
  • STEP 1 - Login to Orion, Set-Up Directory, Locate Tutorial Files
  • STEP 2 - Select the ‘NGS and Sanger Pipeline with Automated Top Lead Selection’ Floe
  • STEP 3 - Prepare Sanger Files
  • STEP 4 - Prepare PacBio Files and Start Job
  • STEP 5 - Open the Floe Report to Get a Detailed Understanding of the Selected or ‘Picked’ Population
  • STEP 6 - Subset NGS Only Population by Cluster and Overlap_Population
• Tutorial 4: NGS and Sanger Pipeline with Automated Top Lead Selection (Illumina), In-Vitro Library
  • Background
  • STEP 1 - Login to Orion, Set-Up Directory, Upload Files
  • STEP 2 - Select the ‘NGS and Sanger Pipeline with Automated Top Lead Selection’ Floe
  • STEP 3 - Prepare Sanger Files
  • STEP 4 - Prepare Illumina Files and Start Job
• Tutorial 5: NGS Pipeline with Automated Top Lead Selection (PacBio), Patient Library
  • Background
  • STEP 1 - Login to Orion, Set-Up Directory, Upload Files
  • STEP 2 - Select the ‘NGS Pipeline with Automated Lead Selection’ Floe
  • STEP 3 - Prepare PacBio Run and Start Job
  • STEP 4 - Select Different Clonotyping Method
  • STEP 5 - Identify both the Picked and the Downstream Population
• Tutorial 6: NGS UMIs Extract and Annotation Floe
  • Background
  • STEP 1 - Login to Orion, Set-Up Directory, Upload Files
  • STEP 2 - Select the ‘NGS Pipeline with Automated Lead Selection’ Floe
  • STEP 3 - Prepare PacBio Run and Start Job
  • STEP 4 - Select Different Clonotyping Method
  • STEP 5 - Identify both the Picked and the Downstream Population
• Video Tutorials Featuring AbXtract

• Index