C2. Cryptic Pocket Detection: Receptor Creation¶
Category Paths
Follow one of these paths in the Orion user interface, to find the floe.
Product-based/Molecular Dynamics
Solution-based/Virtual-screening/Target Preparation
Solution-based/Hit to Lead/Target Preparation/Enhanced Sampling
Solution-based/Target Identification/Target Preparation/Pocket Detection
Solution-based/Hit to Lead/Target Preparation/Cryptic Pocket Detection
Role-based/Computational Chemist
Task-based/Target Prep & Analysis/Pocket Detection
Description
This floe creates design units with receptors constructed from site residues defined for a user specified cryptic pocket and calculates the receptor volume.
Promoted Parameters
Title in user interface (promoted name)
Inputs
Solvated and Equilibrated Design Unit (du_data_in): This is the ‘Solvated and Equilibrated Design Unit’ output dataset from the ‘A1. Protein Sampling (for Cryptic Pockets): Solvate and Equilibrate Target Protein’ floe.
Required
Type: data_source
MSM Weighted Medoids (conf_data_in): This is a ‘MSM Weighted Medoids’ dataset generated by one of the ‘C1. Cryptic Pocket Detection’ Floes: ‘Cooperative Cosolvent Binding Analysis’, ‘Cosolvent Binding Free Energy Analysis’, or ‘Exposon Analysis’.
Required
Type: data_source
Ranked Pockets (pocket_data_in): This is a ‘Ranked Pockets’ dataset generated by one of the ‘C1. Cryptic Pocket Detection’ Floes: ‘Cooperative Cosolvent Binding Analysis’, ‘Cosolvent Binding Free Energy Analysis’, or ‘Exposon Analysis’.
Required
Type: data_source
Pocket Rank ID (pocket_rank): The rank ID number for the pocket of interest. The pocket and its rank ID can be selected by analyzing an ‘interactive network plot of pockets’ in the Floe report generated by one of the ‘C1. Cryptic Pocket Detection’ Floes: ‘Cooperative Cosolvent Binding Analysis’, ‘Cosolvent Binding Free Energy Analysis’, or ‘Exposon Analysis’.
Required
Type: integer
Outputs
Output Dataset (success_data_out): Output dataset to write to
Required
Type: dataset_out
Default: Cryptic Pocket Receptors Dataset
Failure Output Dataset (failure_data_out): Output dataset to write to
Required
Type: dataset_out
Default: Cryptic Pocket Receptors - Failure
Floe Report Output Collection (floe_report_name): File name for the output floe report.
Required
Type: string
Default: Pocket Receptor Volume Floe Report
Advanced Options
Minimum Receptor Volume (volume_min): An lower bound on the receptor volumes of design units that are sent to the output dataset.
Type: decimal
Maximum Receptor Volume (volume_max): An upper bound on the receptor volumes of design units that are sent to the output dataset.
Type: decimal